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For research use only.

Applications: WB,IHC     

Species Cross-Reactivity: H,M,R   

Molecular Weight: ~47KD     

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:2,000 IHC: 1:200

Optimal dilutions should be determined by the end user.

Specificity: The NSE Mouse Monoclonal antibody detects endogenous NSE proteins. 

Background: Enolase is a glycolytic enzyme catalyzing the reaction pathway between 2 phospho glycerate

and phosphoenol pyruvate. In mammals, enolase molecules are dimers composed of three distinct subunits

(alpha, beta and gamma). The alpha subunit is expressed in most tissues and the beta subunit only in muscle.

The gamma subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. NSE

(neuron specific enolase) is found in elevated concentrations in plasma in certain neoplasias. These include

pediatric neuroblastoma and small cell lung cancer. Coexpression of NSE and chromogranin A is

common in neuroendocrine neoplasms

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation   

                            ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep