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For research use only.

Applications: IHC    

Species Cross-Reactivity: H,M,R   

Molecular Weight: N/A     

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: IHC: 1:200

Optimal dilutions should be determined by the end user.

Specificity: The Ki 67 Mouse Monoclonal antibody detects endogenous Ki 67 proteins. 

Background: Antigen KI-67 is a nuclear protein that is associated with and may be necessary for cellular

proliferation. Furthermore it is associated with ribosomal RNA transcription. Inactivation of antigen KI-67

leads to inhibition of ribosomal RNA synthesis. The Ki-67 protein (also known as MKI67) is a cellular marker

for proliferation. It is strictly associated with cell proliferation. During interphase, the Ki-67 antigen can be

exclusively detected within the cell nucleus, whereas in mitosis most of the protein is relocated to the surface

of the chromosomes. Ki-67 protein is present during all active phases of the cell cycle (G 1 , S, G 2 , and

mitosis), but is absent from resting cells (G 0 ).

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation  

                           ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep