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For research use only.

Application:WB

Species Cross-Reactivity: H,R,M

Molecular Weight: 65KD

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:500-2,000

Optimal dilutions should be determined by the end user.

Specificity: NF κ B p65 Mouse monoclonal antibody detects endogenous p65 proteins. 

Background: NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for

termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310

by CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and

impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a

pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of

immune function required for resistance to infection.

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                       ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep