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For research use only.

Application: WB  

Species Cross-Reactivity: H, R, M   

Molecular Weight: 48KD         

Isotype: IgG1

Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.

Store : -20°C. Do not aliquot the antibody.

Recommended dilutions: WB: 1:3,000

Optimal dilutions should be determined by the end user.

Specificity: FH Mouse Monoclonal antibody detects endogenous FH proteins. 

Background: Fumarase (FH) is an enzyme that catalyzes the reversible hydration/dehydration of fumarate

to malate. Fumarase comes in two forms: mitochondrial and cytosolic. The mitochondrial isoenzyme is involved

in the Krebs Cycle (also known as the Tricarboxylic Acid Cycle [TCA] or the Citric Acid Cycle), and the cytosolic

isoenzyme is involved in the metabolism of amino acids and fumarate. Subcellular localization is established

by the presence of a signal sequence on the amino terminus in the mitochondrial form, while subcellular

localization in the cytosolic form is established by the absence of the signal sequence found in

the mitochondrial variety.

Applications:WB-Western blot   IHC-Immunochemistry   IF-Immunofluorescence   IP-Immunoprecipitation

                         ChIP-Chormatin Immunoprecipitation

Reactivity:   H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep