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NF κB p65 Mouse Monoclonal Antibody (14H2)
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NF κB p65 Mouse Monoclonal Antibody (14H2) |
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Catalog |
XHY076M |
XHY076L |
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Quantity |
50µL |
100µL |
For research use only.
Applications: WB, IHC
Species Cross-Reactivity: H,M,R
Molecular Weight: ~65KD
Isotype: IgG1
Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Store : -20°C. Do not aliquot the antibody.
Recommended dilutions: WB: 1:1,000-3,000 IHC: 1:200
Optimal dilutions should be determined by the end user.
Specificity: NFkB p65 Mouse monoclonal antibody detects endogenous p65 proteins
Background: NFkB p65 is ubiquitinated leading to its proteosomal degradation, which is required for
termination of the NFkB response. Phosphorylation of NFkB p65 on S536 stimulates acetylation of K310 by
CBP, enhancing transcriptional activity. NFkB p65 is also acetylated at K122, enhancing DNA binding and
impairing the interaction with NFKBIA. The protein is deacetylated by HDAC3. Invasion of a host by a
pathogen is frequently associated with the activation of NF-kB, which coordinates various aspects of
immune function required for resistance to infection..
Applications:WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep