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PCNA Mouse Monoclonal Antibody(12D10)
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PCNA Mouse Monoclonal Antibody(12D10) |
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Catalog |
XHY044M |
XHY044L |
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Quantity |
50µL |
100µL |
For research use only.
Applications: WB, IHC
Species Cross-Reactivity: H, R, M
Molecular Weight: 29KD
Isotype: IgG1
Storage Buffer & Condition: PBS, pH 7.4, containing 0.02% sodium azide as Preservative and 50% Glycerol.
Store : -20°C. Do not aliquot the antibody.
Recommended dilutions: WB: 1: 5,000, IHC: 1:200
Optimal dilutions should be determined by the end user.
Specificity: Antibody can detects endogenous PCNA protein.
Background: PCNA is a marker for cells in early G1 phase and S phase of the cell cycle. It is found in the
nucleus and is a cofactor of DNA polymerase delta. PCNA acts as a homotrimer and helps increase the
processivity of leading strand synthesis during DNA replication. In response to DNA damage, PCNA is
ubiquitinated and is involved in the RAD6 dependent DNA repair pathway. Two transcript variants
encoding the same protein have been found for PCNA. Pseudogenes of this gene have been
described on chromosome 4 and on the X chromosome.
Applications:WB-Western blot IHC-Immunochemistry IF-Immunofluorescence IP-Immunoprecipitation
ChIP-Chormatin Immunoprecipitation
Reactivity: H-Human R-Rat M-Mouse Mk-Monkey Dg-Dog Ch-Chicken Hm-Hamster Rb-Rabbit Sh-Sheep